Investigation of CRISPR/Cas9-induced SD1 rice mutants highlights the importance of molecular characterization in plant molecular breeding

Sukumar Biswas; Jiaqi Tian; Rong Li; Xiaofei Chen; Zhijing Luo; Mingjiao Chen; Xiangxiang Zhao; Dabing Zhang; Staffan Persson; Zheng Yuan; Jianxin Shi

Journal article

Investigation of CRISPR/Cas9-induced SD1 rice mutants highlights the importance of molecular characterization in plant molecular breeding

Sukumar Biswas, Jiaqi Tian, Rong Li, Xiaofei Chen, Zhijing Luo, Mingjiao Chen, Xiangxiang Zhao, Dabing Zhang, Staffan Persson, Zheng Yuan, Jianxin Shi

JOURNAL OF GENETICS AND GENOMICS | SCIENCE PRESS | Published : 2020

DOI: 10.1016/j.jgg.2020.04.004

Abstract

Although Clustered Regularly Interspaced Short Palindromic Repeats (CRISPR)/CRISPR-associated 9 (Cas9) system has been widely used for basic research in model plants, its application for applied breeding in crops has faced strong regulatory obstacles, due mainly to a poor understanding of the authentic output of this system, particularly in higher generations. In this study, different from any previous studies, we investigated in detail the molecular characteristics and production performance of CRISPR/Cas9-generated SD1 (semi-dwarf 1) mutants from T2 to T4 generations, of which the selection of T1 and T2 was done only by visual phenotyping for semidwarf plants. Our data revealed not only on..

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University of Melbourne Researchers

Staffan Persson Author

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Grants

Awarded by China National Transgenic Plant Special Fund

Awarded by Australian Research Council

Awarded by Programme of Introducing Talents of Discipline to Universities (111 Project)

Funding Acknowledgements

We thank Prof. Jiankang Zhu and Dr. Hui Zhang (Shanghai Normal University) for providing pBIN-sgR-Cas9-OsU3 vector. This work was supported by China National Transgenic Plant Special Fund (2016ZX08012-002, 2016ZX08009-003-007 and 2017ZX08013001-001) to DZ, JS, and ZY, respectively; SMC Morningstar Young Scholarship of Shanghai Jiao Tong University to ZY; the Australian Research Council (DP19001941, FT160100218) and an IRRTF grant from UoM to SP; and the Programme of Introducing Talents of Discipline to Universities (111 Project, B14016) to DZ.